By Sudha R. Kini MD
Written by means of a number one specialist in thyroid biopsy interpretation, this text/atlas is the main finished reference on thyroid pathology. It includes over 1,700 full-color photomicrographs depicting the total variety of findings obvious on superb needle aspiration biopsies. The illustrations exhibit the vast spectrum of cytologic positive factors for every disorder entity, together with strange in addition to standard styles. specific realization is given to diagnostic pitfalls, overlapping styles, and differential analysis. The publication additionally contains chapters on simple recommendations of cytopathology, fine-needle aspiration biopsy thoughts, cytopreparation, ancillary diagnostic recommendations, liquid-based cytologic arrangements, and the appliance of needle biopsy findings to administration decisions.
The absolutely searchable on-line textual content and a full-color photograph financial institution can be found on a better half Website.
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Additional info for Thyroid cytopathology : an atlas and text
If the sediment is still very bloody, repeat the above procedure. Saponin technique should not be used more than twice, as it may lyse the cells of diagnostic importance. Any leftover sediment is further processed for a cell block (see below). CYTOCENTRIFUGATION AND CYTOSPIN PREPARATION Cytospin preparation is the method of choice for harvesting poorly cellular samples. Cytocentrifugation allows the concentration of cells on a glass slide by way of collecting them over a filter placed on the "well" etched on a special glass slide manufactured for the procedure.
Dip smears 5 times in solution II, 1 second each time; allow excess to drain. Rinse the smears in distilled water. Check the quality of the stain before coverslipping. If necessary, restain in Solutions I and II for one or two seconds. Allow the slides to dry. Coverslip. Heme-3 Stain Reagents Heme-3 fixative Heme-3 solution I Heme-3 solution II Distilled water Procedure Dip air-dried smears in fixative 5 times, 1 second each time; allow excess to drain. Dip smears 5 times in solution I, 1 second each time; allow excess to drain.
Number of Smears If a smear looks unsatisfactory, it usually is. If it looks satisfactory, it may be so. We take six smears to ensure three good ones and adequate sampling. FIXATION Fixation must be matched to the staining technique employed. We use a modified Papanicolaou staining technique and therefore fix immediately with alcohol as part of a spray. If Mayâ€“GrÃ¼nwaldâ€“Giemsa stain is used, the smear is air dried and no prompt fixation is necessary. POSTBIOPSY CARE If there has been no intranodular bleeding, we apply an elastic bandage and keep the patient under surveillance for about 15 minutes.
Thyroid cytopathology : an atlas and text by Sudha R. Kini MD