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Download PDF by Shih Yi Wang: Photochemistry and Photobiology of Nucleic Acids. Biology

By Shih Yi Wang

ISBN-10: 0127346023

ISBN-13: 9780127346021

ISBN-10: 0323150039

ISBN-13: 9780323150033

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Extra resources for Photochemistry and Photobiology of Nucleic Acids. Biology

Example text

Studier, F. W. (1965). /. Mol. Biol. 11, 373. Sutherland, Β. , and Sutherland, J. C. (1969). Biophys. J. 9, 292. Szybalski, W. (1968). In "Methods in Enzymology" (L. Grossman and K. ), Vol. 12, Part B, p. 330. Academic Press, New York. Thomas, C. , (1956). /. Amer. Chem. Soc. 78, 1861. Tunis-Schneider, M. , and Hearst, J. (1968). Biopolymers 6, 1218. Tunis-Schneider, M. , and Maestre, M. F. (1970). /. MoJ. BioJ. 52, 521. , and Hearst, J. E. (1962). Progr. Chem. Org. Natur. Prod. 20, 373. , and Britten, R.

So as a first approximation at least, we can neglect inter­ nucleotide interactions and talk in terms of photon absorption by a single base in DNA. Nevertheless, we must keep in mind that the con­ straints imposed on intranucleotide interaction by the double helical conformation can influence de-excitation processes and, thus, the ul­ timate photochemistry of the DNA bases. Furthermore, because of their higher energy, bases in excited states have much stronger in­ teractions than they do in the ground state.

Acid Hydrolysis DNA can be hydrolyzed to its component Pur and Pyr bases, plus acid-stable photoproducts, by heating in acid: usually either triflouracetic (TFA), formic (FA), or perchloric (PA) acid. The first two acids are preferred when paper chromatography analysis is used, since their high vapor pressures allow complete removal of the acid prior to application of the hydrolysate to the paper. 0 ml TFA in a thick-walled Pyrex tube. The tube is sealed, placed in a container (preferably metal in order to withstand possible explosive breakage), and heated 90 min at 170°-175°C.

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Photochemistry and Photobiology of Nucleic Acids. Biology by Shih Yi Wang

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