By Robert Peter Gale, Jean-Louis Touraine, Vinod Kochupillai (auth.), Jean-Louis Touraine, Robert Peter Gale, Vinod Kochupillai (eds.)
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The fibrotic pancreas remnant was infiltrated by mononuclear cells in several cases. However, no clearcut difference was found between recipients of DLA-identical and DLAhaploidentical grafts. Long-term survivors were free of alterations resembling GvHD. When tested in MLC, circulating lymphocytes of recipients given homozygous, DLA-haploidentical grafts were tolerant to host antigens both early and late after FLT (Table 8). Discussion The present study confirms earlier findings [15-17] indicating that cryopreserved canine FLC are a potent source of pluripotent hemopoietic stem cells able to restore hemopoiesis and immune functions in adult recipients previously rendered aplastic by high-dose TBl.
Irradiated dogs reconstituted with fetal liver cells recovered mitogen responsiveness within 3 months; MLC reactivity recovered less 51 rapidly but relatively few animals have been studied [28, 29, 32, 33]. Data in dogs from ourselves and Prummer and coworkers suggest that B-cell recovery preceeds T-cell recovery. This was unanticipated since, in man, T-cell rather than B-cel1 engraftment is more easily achieved [27, 40, 71]. These differences may be species specific or may be related to either DLA-matching between donor and recipient or to the fact that recipients were irradiated and therefore achieved complete hematopoietic grafts rather than only lymphoid grafts.
Since splenic CFU~GM pools were severely depressed in most of the animals, extramedullary hemaopoiesis in the spleen could not account for the expanded pool of circulating progenitors. An early peak of the colony-stimulating activity in serum 3~ 7 days after FLT coincided with the initial recovery phase of granulopoiesis and monocytopoiesis and was not paralleled by elevated serum endotoxin levels. M egakaryopoiesis In bone marrow aspirates, megakaryocytes were normally detected on days 7 or 10 after FLT.
Fetal liver transplantation by Robert Peter Gale, Jean-Louis Touraine, Vinod Kochupillai (auth.), Jean-Louis Touraine, Robert Peter Gale, Vinod Kochupillai (eds.)