By Ricco Palmeri, Sal Grimaudo
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Additional resources for Estradiol: Synthesis, Health Effects and Drug Interactions
Extraction Extraction of steroid sex hormones and related synthetic compounds from environmental water is usually performed by offline solid-phase extraction (SPE). Given the low analyte concentrations found in water samples, a pre-concentration by SPE is required prior to analysis. SPE now belongs to well established methods due to the experimental simplicity, easy automation, a smaller consumption of nonaqueous solvents compared to conventional techniques, and to availability of many different sorbents.
2007) A real-time reverse transcriptase PCR (RT-PCR) assay was developed for quantification of VTG mRNA level in both control fish and E2-treated fish. 1 g/L for medaka in a 2-day exposure experiment. Further kinetics studies of the two fish models indicated that medaka was able to respond much faster to E2 treatment than zebrafish, while the zebrafish can attain a much higher level of VTG mRNAs than medaka after a long-term E2 treatment. , 2004). Quantitative real time PCR (Q-RT-PCR) is a new method for analyzing gene expression with several advantages such as speed, directness, and accuracy.
Proposal for a DIRECTIVE OF THE EUROPEAN PARLIAMENT AND OF THE COUNCIL amending Directives 2000/60/EC and 2008/105/EC as regards priority substances in the field of water policy. pdf. , 2000. do? uri=OJ:L:2000:327:0001:0072:EN:PDF. , 2001. DECISION No 2455/2001/EC -establishing the list of priority substances in the field of water policy and amending Directive 2000/60/EC. uri=OJ:L:2001:331:0001:0005:EN:PDF. , 2008a. uri=OJ:L:2008:348:0084:0097:EN:PDF. , 2008b. DIRECTIVE 2008/105/EC - on environmental quality standards in the field of water policy, amending and subsequently repealing Council Directives 82/176/EEC, 83/513/EEC, 84/156/EEC, 84/491/EEC, 86/280/EEC and amending Directive 2000/60/EC of the European Parliament and of the Council.
Estradiol: Synthesis, Health Effects and Drug Interactions by Ricco Palmeri, Sal Grimaudo