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Antonia Vlahou, Manousos Makridakis's Clinical Proteomics: Methods and Protocols PDF

By Antonia Vlahou, Manousos Makridakis

ISBN-10: 1493918710

ISBN-13: 9781493918713

ISBN-10: 1493918729

ISBN-13: 9781493918720

This moment editionexpands upon the former version with present, distinctive advancements within the box and brings jointly a multi-disciplinary workforce of prime researchers to supply their most up-to-date protocols for scientific proteomics research. Written within the hugely profitable Methods in Molecular Biology sequence layout, chapters comprise introductions to their respective issues, lists of the required fabrics and reagents, step by step, simply reproducible laboratory protocols, and pointers on troubleshooting and warding off recognized pitfalls.

Authoritative and functional, Clinical Proteomics: equipment and Protocols, moment Edition offers techniques that might function a competent advisor to researchers, together with clinicians, chemists, molecular biologists, bioinformaticians and computational, biologists, and investigators engaged on biomarker development.

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Extra info for Clinical Proteomics: Methods and Protocols

Sample text

Rabouille C, Malhotra V, And Nickel W (2012) Diversity in unconventional protein secretion. J Cell Sci 125:5251–5255 3. Haslene-Hox H, Tenstad O, And Wiig H (2013) Interstitial fluid-a reflection of the tumor cell microenvironment and secretome. Biochim Biophys Acta 1834:2336–2346 4. Kupcova Skalnikova H (2013) Proteomic techniques for characterisation of mesenchymal stem cell secretome. Biochimie 95(12):2196–2211 5. Pellitteri-Hahn MC, Warren MC, Didier DN et al (2006) Improved mass spectrometric proteomic profiling of the secretome of rat vascular endothelial cells.

Humana, Totowa Body Fluids-SOPs 28. Casado B, Iadarola P, Pannell L (2008) Preparation of nasal secretions for proteome analysis. In: Posch A (ed) 2D PAGE: sample preparation and fractionation. Humana, Totowa, pp 77–87 29. Caseiro A, Ferreira R, Padrao A et al (2013) Salivary proteome and peptidome profiling in type 1 diabetes mellitus using a quantitative approach. J Proteome Res 12(4): 1700–1709 30. Casado B, Iadarola P, Pannell LK (2008) Preparation of nasal secretions for proteome analysis.

28. If the collected amniotic fluid contains blood, proteomic analysis of the specimen may be impaired [71]. 1 Introduction Bile is the fluid that is constantly produced by the liver and is drained by biliary ducts into the gallbladder, where it is stored and concentrated. ) compounds. Bile is composed of salts, lipids, proteins, hormones, inorganic ions, and other metabolites [74, 75]. Proteomic analysis of the bile can yield valuable information about pathologies involving the biliary tract. Specifically, recent studies have focused on the discovery of biomarkers for cholangiocarcinoma and pancreas adenocarcinoma.

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Clinical Proteomics: Methods and Protocols by Antonia Vlahou, Manousos Makridakis


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