By Theodore Provder, Marek W. Urban, Howard G. Barth
Read or Download Chromatographic Characterization of Polymers: Hyphenated and Multidimensional Techniques (Advances in Chemistry Series 247) PDF
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Additional info for Chromatographic Characterization of Polymers: Hyphenated and Multidimensional Techniques (Advances in Chemistry Series 247)
Field and Gradient Combinations Effective Property Gradient of the Carrier Liquid Coupled with the Field Action (Isoperichoric Focusing). Focusing can appear as a consequence of the effective property gradient of the carrier l i q u i d i n the direction across the channel combined w i t h the primary or secondary transversal field action. T h e density gradient i n sedimentation-flotation focusing F F F or the p H gradient i n isoelectric focusing F F F were already implemented (2, 3). Preformed Gradient Combined with the Field Action.
T h e other experiments were performed under steady-state conditions determined for each particular case. Because of the preliminary character of the experiments, the quantitative comparison of the experimental results w i t h the theoretical estimations was not relevant. Another experiment was intended to compare the shape of the density gradient formed because of either centrifugation or coupling of electric and gravitational fields i n T L F (13). The positions of the zones were almost identical for both experiments.
T h e results are represented i n F i g u r e 2 as the shapes of several focused zones. T h e important conclusion from this model calculation is that the rigorous or Gaussian distribution functions are almost identical for narrow zones focused at lower x values. H o w e v e r , the shapes of the focused zones are substantially different for broader zones focused at higher x values i f the calculation is per formed either for rigorous or Gaussian distribution function. 005), but it exhibits a maximum.
Chromatographic Characterization of Polymers: Hyphenated and Multidimensional Techniques (Advances in Chemistry Series 247) by Theodore Provder, Marek W. Urban, Howard G. Barth