By Adam E. Golan
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Extra resources for Cellulase : types and action, mechanism, and uses
Zymogram analysis revealed that additional cellulases are produced by T. fusca. , 2004]. 2 and 77°C, respectively. It retained more than 60% of the maximal activity after 24 h incubation at 60°C. The temperature optimum of 77°C is comprised in the temperature optimum range of the other endoglucanase, endoxylanase and endomannanase enzymes from T. fusca (70-80°C), suggesting that the new endoglucanase has similar advantages for industrial application as the other thermostable hydrolases from this thermophilic microorganism.
In order to obtain high-purity cellulase and facilitate its production, the cel9 gene was recently expressed in E. , 2010a]. 5 and 65°C, respectively. , 2010b] are somewhat surprising, but can be explained by possible acquisition of the cel9–cel48 gene cluster from a thermophilic microorganism through horizontal gene transfer. 2 Fungal Cellulases Fungal cellulases are well-studied enzymes used in various industrial processes [Bhat, 2000], and the properties of several of them, not considered in this text, are listed in Table 5.
8 and 50°C. A similar optimal temperature was reported for enzymes from Melanocarpus sp. , 2007]. When heated at 50°C for 1 h, the endoglucanase from A. , 2004]. , 2010]. 0 and 50°C, respectively. 5) and at temperatures below 55 °C. It retained only 60% activity after incubation at 60°C for 1 h. The newly isolated endoglucanase from A. glaucus XC9 shares common characteristics with those from industrial cellulaseproducing Fungi, such as A. niger and T. reesei suggesting its possible use in industry.
Cellulase : types and action, mechanism, and uses by Adam E. Golan