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Cellulase : types and action, mechanism, and uses by Adam E. Golan PDF

By Adam E. Golan

ISBN-10: 1611222559

ISBN-13: 9781611222555

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Extra resources for Cellulase : types and action, mechanism, and uses

Example text

Zymogram analysis revealed that additional cellulases are produced by T. fusca. , 2004]. 2 and 77°C, respectively. It retained more than 60% of the maximal activity after 24 h incubation at 60°C. The temperature optimum of 77°C is comprised in the temperature optimum range of the other endoglucanase, endoxylanase and endomannanase enzymes from T. fusca (70-80°C), suggesting that the new endoglucanase has similar advantages for industrial application as the other thermostable hydrolases from this thermophilic microorganism.

In order to obtain high-purity cellulase and facilitate its production, the cel9 gene was recently expressed in E. , 2010a]. 5 and 65°C, respectively. , 2010b] are somewhat surprising, but can be explained by possible acquisition of the cel9–cel48 gene cluster from a thermophilic microorganism through horizontal gene transfer. 2 Fungal Cellulases Fungal cellulases are well-studied enzymes used in various industrial processes [Bhat, 2000], and the properties of several of them, not considered in this text, are listed in Table 5.

8 and 50°C. A similar optimal temperature was reported for enzymes from Melanocarpus sp. , 2007]. When heated at 50°C for 1 h, the endoglucanase from A. , 2004]. , 2010]. 0 and 50°C, respectively. 5) and at temperatures below 55 °C. It retained only 60% activity after incubation at 60°C for 1 h. The newly isolated endoglucanase from A. glaucus XC9 shares common characteristics with those from industrial cellulaseproducing Fungi, such as A. niger and T. reesei suggesting its possible use in industry.

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Cellulase : types and action, mechanism, and uses by Adam E. Golan

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