By Rene A. Frenkel
ISBN-10: 0122670604
ISBN-13: 9780122670602
ISBN-10: 0323153402
ISBN-13: 9780323153409
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Additional info for Carnitine Biosynthesis, Metabolism, and Functions
Example text
And Tugendhaft-Mills, J. (1978) J. Lipid Res. 19, 1057-1063. DISCUSSION A. SHJLTG» Could you comment on a recent paper which indicates that in the guinea-pig about 50% of the carnitine synthesized is made in the kidney? R» FRENKEL: I am not aware of the specific paper you mention, but I do know that the enzymes required for the complete synthesis of carnitine have been reported to be present in the kidneys of several species. L. HENDERSON: If one accepts the idea that trimethyllysine comes from protein-bound trimethyllysine, how would it get to the kidney since it is taken up reluctantly by this organ if present in the plasma?
L i n d s t e d t , S . , and R u n d g r e n , M . J . B i o l . C h e m . 252:5073 (1977). L i n d s t e d t , S . , O d e l h ö g , Β . , and R u n d g r e n , M . B i o c h e m i s t r y 16:3369 (1977). O h n i s h i , T . , H i r a t a , F . , a n d H a y a i s h i , O . J . B i o l . C h e m . 252:4643 (1977). K i d e , T . , a n d S o d a , K . J . B i o l . C h e m . 253:226 (1978). L i n d s t e d t , G . B i o c h e m i s t r y 6:1271 (1967). E r f l e , J . D . B i o c h e m . B i o p h y s .
In vitro" conversion of TML to Hydroxytrimethyllysine in Kidney Mitochondria Kidney mitochondria were incubated under air at 37° with the complete hydroxylase system. Aliquots were withdrawn at the times indicated and the metabolites separated by HPLC. 28 CARNITINE BIOSYNTHESIS, METABOLISM, AND FUNCTIONS BB FRACTION NUMBER Figure 7. "In vivo" conversion to TML to products. An aliquot of an extract prepared from rat kidney 30 minutes after the injection of 5 ymoles of L-[methyl-^H] TML was analyzed by HPLC and the radioactivity in the different metabolites measured in a scintillation counter.
Carnitine Biosynthesis, Metabolism, and Functions by Rene A. Frenkel
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