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Download e-book for kindle: Carbohydrate Metabolism - Part D by Willis A. Wood (Eds.)

By Willis A. Wood (Eds.)

ISBN-10: 0121819892

ISBN-13: 9780121819897

The seriously acclaimed laboratory general, Methods in Enzymology, is without doubt one of the so much hugely revered courses within the box of biochemistry. because 1955, every one quantity has been eagerly awaited, usually consulted, and praised through researchers and reviewers alike. The sequence includes a lot fabric nonetheless suitable this present day - actually a necessary book for researchers in all fields of existence sciences

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Extra info for Carbohydrate Metabolism - Part D

Sample text

Alcohol Membrane-bound alcohol dehydrogenase of the acetic acid bacteria catalyzes oxidation of ethanol in vivo, and the enzyme activity is linked to the electron transport system of the organisms. The enzyme catalyzes the reaction in vitro, and the rate of alcohol oxidation is measured by following the reduction of dye. 0, for the enzyme from Acetobacter species. This buffer is prepared as shown in D-gluconate determination. 0, for the enzyme from Gluconobacter species. This buffer is prepared as mentioned above (D-fructose determination).

Shinagawa, O. Adachi, and M. Ameyama, Agric. Biol. Chem. 44, 1505 (1980). 1 M KCI 1% Triton X-100 1% Triton X-100 + 1 M KC1 1 2 3 4 5 c c 6 7 8 9 10 11 12 a Numbers refer to text footnotes. b DOC, deoxycholate. c M. Ameyama, unpublished. u n d e r t a k e n . O x i d a t i o n o f i n d i v i d u a l s u b s t r a t e s is l i n k e d to t h e r e s p i r a t o r y c h a i n , a n d t h e r e a c t i o n r u n s to c o m p l e t i o n , S i n c e t h e s e e n z y m e s a r e at t h e culture interface, where a large amount of sugar acids accumulates, they a r e f a i r l y s t a b l e at a c i d i c p H a n d t h e o p t i m u m p H is o f t e n in t h e a c i d i c regions.

J. Hochellaand S. Weinhouse,Anal. Biochern. 10, 304 (1965). METHODS IN ENZYMOLOGY, VOL. 89 Copyright © 1982by Academic Press, Inc. All rights of reproduction in any form reserved. ISBN 0-12-181989-2 30 ANALYTICAL METHODS [5] variable and not completely specific for lactic acid. 2"4 While other modifications of the original colorimetric method are widely used 4-6 to eliminate pyruvic acid and other interfering substances, it is interesting that no color development occurs if a high-purity grade of H2SO4 is used, 4 suggesting a catalytic effect of trace impurities.

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Carbohydrate Metabolism - Part D by Willis A. Wood (Eds.)

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