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By A. Fiechter (auth.)

ISBN-10: 0387135391

ISBN-13: 9780387135397

ISBN-10: 3540135391

ISBN-13: 9783540135395

ISBN-10: 3540390049

ISBN-13: 9783540390046

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5 ml * Solution, mg 1-1 A final e x a m p l e o f t h e i m p o r t a n c e o f c o r r e c t m e d i u m d e s i g n is t h e a p p l i c a t i o n o f d e f i n e d b i o s y s t e m s f o r t e s t i n g a e r a t i o n a n d a g i t a t i o n h a r d w a r e . T h e b a s i c idea in s u c h a n a p p r o a c h is t h e fact t h a t c h e m i c a l l y d e f i n e d m e d i a s u p p o r t biologically defined regulation of growth and product formation. Variation of medium concent r a t i o n s (So) p r o v i d e s t h e p o s s i b i l i t y o f c o r r e c t l y a d j u s t i n g t h e b i o s y s t e m to t h e m a s s t r a n s f e r c a p a c i t y o f t h e b i o r e a c t o r (Fig.

Much of it has been summarized by S. A. Koser 48~. Amino acids, or building blocks for protein synthesis, must be added to media when auxotrophic strains are used or when the potential of amino acid s~cnthesis is restricted. The latter case creates problems in proper identification of the actual bottleneck due to the complicated regulation patterns in these branched, biosynthetic pathways. In many cases, media development does not meet the final requirements of a synthetic solution because complete identification of all essential compounds is not possible.

The flow pattern can be kept under perfect control regardless of the actual gas hold-up. 4 m 3 Fig. 10. Jet loop reactor (JLR) (by Blenke 26)). The tall form (height: diameter ~ 10) provides an extended residence time. Consequently, the air at exit is well depleted of oxygen. A rather large head-space is needed to prevent overflow of foam G De Physical and Chemical Parameters of Microbial Growth G G~ r I DQ DT V 250 m m 900 m m 400 t 27 Fig. 11. Annular form (TORUS) with mechanical drive (see Laederach 3o)).

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Bioprocess Parameter Control by A. Fiechter (auth.)


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