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Michael Barany's Biochemistry of Smooth Muscle Contraction PDF

By Michael Barany

ISBN-10: 0120781603

ISBN-13: 9780120781607

This invaluable source offers a scientific account of the biochemistry of tender muscle contraction. As a entire consultant to this speedily transforming into sector of analysis, it covers the constitution and attribute homes of contractile and regulatory proteins, with designated emphasis on their estimated functionality within the stay muscle. additionally incorporated during this publication are intermediate filament proteins, and desmin and vimentin, whose functionality in delicate muscle is unknown; and a number of other enzymes enthusiastic about the phosphorylation-dephosphorylation of contractile and different proteins

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Additional info for Biochemistry of Smooth Muscle Contraction

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8 _.. M-D 9 ~ 10 _.. M ~" kL--Minor Effect Strong Binding States F I G U R E 8 Kinetic scheme for the hydrolysis of ATP by s m o o t h muscle actomyosin. M = m y o s i n (or its subfragments); A = actin; T = ATP; D = ADP; Pi = inorganic p h o s p h a t e . Measurement of individual rate constants within this cycle demonstrates the kinetic basis for the regulation of actin-activated MgATPase activity of myosin by actin. P i complex. 002 s -] in the absence of actin and at an identical rate in the presence of 5 ~M actin (Sellers, 1985).

The chicken smooth muscle myosin sequence has an insert of 7 amino acids starting at residue 209 (see text) and the sequence has been corrected according to Kelley et al. (1993). The rabbit smooth muscle sequence is from uterus and does not contain the inserted amino acids. (For the amino acid sequence of HC200 isoforms, see Fig. ) Regions of very strong homology among the four proteins are boxed. The regions corresponding to loop I or loop 2 are overlined and labeled. , 1993b). The start of the 25-, 50-, 20-kDa, S-2, and LMM regions are indicated, as are the LC17 and LC20 binding regions.

Myosin Structure and Function Insertion NH2q 25kDT 50kD Head |5 COOH200 12OkDI III- COOH204 Rod Chicken Gizzard H K G K K D T S I T ......... QGPSFSYGELEKQLLQA Rabbit Visceral ............. LA Rat Visceral S Chicken Aorta Rabbit Vascular Rat Vascular . . . S _ _ _ Chicken NMHC-B - - - R - - H N - P P E S P K P V K H ....... Xenopus NMHC-B . . . H T - P ~ . . A I - - - S G - L L . . . . . - - - - - - o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o A o o o o o o o o o o o o o o o o FIGURE 7 Diagrammatic representation of the smooth muscle HC to show the location of the 7-amino-acid insert.

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Biochemistry of Smooth Muscle Contraction by Michael Barany


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