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Read e-book online Advances in lipid research. / Volume 19 PDF

By Rodolfo Paoletti, Dr. David Kritchevsky

ISBN-10: 0120249197

ISBN-13: 9780120249190

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063 from normal intima and plaque homogenates. These particles had a somewhat more heterogeneous size distribution than plasma LDL; they reacted with apoB antiserum but showed an electrophoretic mobility different from that of apoB, being more anodic than plasma LDL. The apoprotein moieties appear to include, besides apoB, albumin and possibly apoA-I and apoC peptides. The lipid composition of the lipoproteins from normal intima closely resembled that of plasma LDL; however, plaque particles contained more free cholesterol than plasma LDL.

Sirtori et al. (1976) conducted experiments designed to study in vivo and in vitro the inhibition of lipoprotein interactions with arterial components caused by a sulfated mucopolysaccharide of duodenal origin (3-GS). In the in vivo assays, rabbits were given an intravenous dose of radioiodinated hypercholesterolemic VLDL, and 1 and 2 hours after the injection the radioactivity present in the aortic intima-media was measured. In all cases, the animals receiving a continuous infusion of the duodenal polysaccharide deposited less radioactivity in aorta.

3. , 1976, 1980). This sample was prepared by affinity chromatography on an agarose-LDL column. The sample was negatively stained with phosphotungstic acid, x 54,225. 1 /mi. (B) Mixture of human plasma LDL and the lipoprotein-complexing proteoglycan (LCP) showing the large aggregates of LDL apparently aligned along fibrous structures, x 43,875. 1 /-on. (C) Control preparation; the same human LDL as in B, but mixed with a sample of LCP that has been preincubated with chondroitinase AC. x 43,875.

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Advances in lipid research. / Volume 19 by Rodolfo Paoletti, Dr. David Kritchevsky


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